(p)ppGpp, through its interaction with RNA polymerase, aids in managing replication conflicts and facilitates the loading of ...

Science DNA breaks are toxic lesions for the cell. In order to repair these breaks, it is necessary, starting from the break ends, to find a homologous sequence within the vastness of the genome. In ...

Solving a 30-year mystery Boulton became interested in dynamic single-molecule analysis when studying RAD51 — an enzyme regulated by BRCA2 to repair double-strand DNA breaks. Multiple RAD51 ...

The newly synthesized DNA (light blue) can then be joined with the 5' end of the resected strand, forming a double Holliday junction (double-strand break repair; DSBR), or can be displaced and ...

Homologous recombination plays essential roles in the repair of DNA double strand breaks (DSBs) and collapsed replication forks. The fundamental reaction in homologous recombination is the exchange of ...

For cytoplasmic injections the mix will contain the guide RNA(s), DNA repair template(s), and Cas9 mRNA in nuclease-free buffer at concentrations determined by the core. For pronuclear microinjections ...

The team initially set out to study another replication machinery protein, single-stranded DNA-binding protein ... whether the enzymatic period was during exonuclease repair or DNA extension. “This ...

DNA ligases IIIa (922 amino acids) and IIIb (862 amino acids) are the products of a single gene; they differ in amino ... a 911-amino acid polypeptide that plays a role in the repair of double-strand ...

cells have evolved various DNA repair mechanisms. These mechanisms can detect and correct different types of DNA damage, such as base mismatches, single-strand breaks, and double-strand breaks. DNA ...